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DOI: 10.1016/j.molp.2015.04.007 Corpus ID: 25184623; A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. @article{Ma2015ARC, title={A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.}, …
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A robust CRISPR/Cas9 vector system for multiplex genome editing in monocot and dicot plants was developed. Multiple sgRNA expression cassettes can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning. This system can uniformly, efficiently, and simultaneously produce multiple heritable mutations in rice and …
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A convenient and high-efficiency Agrobacterium rhizogenes-mediated hairy root transformation for CRISPR/Cas9-based gene editing efficiency evaluation in different Citrullus species. ... To rapidly select efficient single guide RNAs (sgRNAs) in the genus watermelon, which is difficult to genetically transform, the Agrobacterium rhizogenes ...
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Base-catalyzed chemical etching of silicon in water can produce hydrogen and dissociated orthosilicic acid (SiO 2 (OH) 2 2−), suggesting that silicon can be regarded as an energy carrier.However, this process needs a large amount of low-priced silicon as the essential reactive material for cost saving and faster and high-yield hydrogen production …
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Fringe projector profilometry (FPP) is an important three-dimensional (3D) measurement technique, especially when high precision and speed are required. Thus, theoretical interrogation is critical to provide deep understanding and possible improvement of FPP. By dividing an FPP measurement process into four steps (system calibration, phase …
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Convenient Syntheses and Versatile Functionalizations of Isotactic Polypropylene Containing Plentiful Pendant Styrene Groups with High Efficiency @article{Wang2015ConvenientSA, title={Convenient Syntheses and Versatile Functionalizations of Isotactic Polypropylene Containing Plentiful Pendant Styrene …
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High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System. Liuhua Yan 1,3 ∙ Shaowei Wei 1,2,3 ∙ Yaorong Wu 1 ∙ ... A Robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol. Plant. 2015; 8:1274-1284. Full Text. Full Text (PDF) …
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A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, …
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A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Xingliang Ma 1,4, Qunyu Zhang 2, Qinlong Zhu 2,4, …
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At present, the application of this method to quaternary ammonium modified straw has not been reported. Herein, a one-step, high-speed shear method (HSSM) was proposed to prepare an efficient, convenient, nontoxic, and green aqueous nitrate adsorbent with high adsorption capacity.
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A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, …
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Fringe projection profilometry method with high efficiency, precision, and convenience: theoretical analysis and development
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Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot …
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